RanBPM shRNA HeLa Cell Line as a Research Tool

Summary

Researchers at Western University have developed stable RanBPM shRNA HeLa cell line as a research tool.

Background

Ran-Binding Protein M (RanBPM) has been previously shown to inhibit c-Raf expression, however how this was achieved remains unclear. c-Raf is the central component of the extracellular signal-regulated kinase (ERK) pathway which has been linked to many cancer types. Furthermore, RanBPM was recently identified as part of the E3 ubiquitin ligase complex and the CTLH (C-terminal to LisH) complex (McTavish et al., 2019). Lastly, RanBPM has been linked to various signaling pathways related to numerous cellular processes which include – apoptosis, cell adhesion, migration, transcription, nuclear-cytoplasmic transport and also plays a significant role during development (Salemi et al., 2017)

Technology Overview

Researchers at Western University have developed a RanBPM shRNA expressing stable HeLa cell line. The RanBPM shRNA expressing stable cell line has been validated and displays complete depletion/knockdown of RanBPM (Figure 3D, McTavish et al., 2019).

The current cell line serves as an excellent reagent or tool to study the functional role of RanBPM in different cellular processes.

Applications

• RanBPM shRNA stable cell line can be used in studies investigating the role of RanBPM and interacting partners
• Delineate pathways RanBPM is associated and involved with
• Available as research tool/reagent for licensing

Opportunity

• Licensing

References

Salemi LM, Maitland MER, McTavish CJ, Schild-Poulter C. Cell signalling pathway regulation by RanBPM: molecular insights and disease implications. Open Biol. 2017;7(6):170081. doi:10.1098/rsob.170081 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5493780/

McTavish, C., Bérubé-Janzen, W., Wang, X., Maitland, M., Salemi, L., Hess, D. and Schild-Poulter, C. (2019). Regulation of c-Raf Stability through the CTLH Complex. International Journal of Molecular Sciences, 20(4), p.934. https://www.mdpi.com/1422-0067/20/4/934

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